Difference between revisions of "Part:BBa K1194004"
 
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<DIV class='curation y2014'>
 
<DIV class='curation y2014'>
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Registry curation: Circular specification
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Circular specification. Registry curation 4-14-2014
  
 
This part was defined with itself as the last subpart.<BR>
 
This part was defined with itself as the last subpart.<BR>
−
That subpart was deleted to make the specification legal. A better correction may be possible.
+
We have reverted this part to a basic part with its original sequence. A different change may be better.
 
</div>
 
</div>
  

Latest revision as of 19:06, 14 April 2014

pLuxR --> ompF --> Gb3 mimic

Gb3 mimic codes for a nine-amino acid peptide that has anti-Shiga toxin activity. It is coded downstream of ompF which is an periplasmic translocation signal peptide. The entire assembly is under control of an N-acyl homoserine lactone (AHL) dependent pLuxR promoter. The part, when translated, produces the Gb3 mimic peptide. The ompF signal peptide helps to transport Gb3 mimic into the extracellular space. Gb3 mimic neutralises the Shiga toxin by binding to it and sequestering it. Other teams can utilise the ompF signal peptide if they want to ensure extracellular secretion of their protein of interest, especially if it is a small peptide. The Gb3 mimic peptide, though specific for the Shiga toxin, can be used for toxicology studies in general. Particularly, it can be of use to create a peptide library to investigate anti-toxin activities of therapeutic peptides by molecular docking.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Circular specification. Registry curation 4-14-2014

This part was defined with itself as the last subpart.
We have reverted this part to a basic part with its original sequence. A different change may be better.