Difference between revisions of "Part:BBa M45136:Design"

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===References===
 
===References===
 
http://www.proteopedia.org/wiki/index.php/3svl
 
http://www.proteopedia.org/wiki/index.php/3svl
 +
 
Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082
 
Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082
  
 
Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179
 
Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179

Latest revision as of 16:32, 14 April 2014


ChrR30, Chromate and Uranium Reductase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 327
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ChrR30 and ChrR6 are improved by directed evolution.


Source

The source of this gene sequence is E. Coli. ChrR and ChrR6 were formerly referred to as YieF protein and Y6. ChrR6 and ChrR30 are the evolved forms of ChrR.

References

http://www.proteopedia.org/wiki/index.php/3svl

Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082

Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179