Difference between revisions of "Part:BBa K245046"
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<partinfo>BBa_K245046 short</partinfo>
 
<partinfo>BBa_K245046 short</partinfo>
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'''''<center><span style="font-weight:bold; font-size:110%; color:red">Due to patent issues, samples of parts containing the ccdB gene cannot be requested. - iGEM Headquarters</span></center>'''''
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All of RFC 37 vectors (BB-NIC-II-HisN - BBa_K245005, BB-NIC-II-NC - BBa_K245006, BB-NIC-II-HisC - BBa_K245007 and BB-NIC-III-HisN - BBa_K245008) have a ccdB gene inserted in their multiple cloning site. CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site as the ones listed above, cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. This part comprises only active ccdB part without inactive ccdA.
 
All of RFC 37 vectors (BB-NIC-II-HisN - BBa_K245005, BB-NIC-II-NC - BBa_K245006, BB-NIC-II-HisC - BBa_K245007 and BB-NIC-III-HisN - BBa_K245008) have a ccdB gene inserted in their multiple cloning site. CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site as the ones listed above, cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. This part comprises only active ccdB part without inactive ccdA.

Revision as of 15:14, 25 March 2014

ccdB


Due to patent issues, samples of parts containing the ccdB gene cannot be requested. - iGEM Headquarters


All of RFC 37 vectors (BB-NIC-II-HisN - BBa_K245005, BB-NIC-II-NC - BBa_K245006, BB-NIC-II-HisC - BBa_K245007 and BB-NIC-III-HisN - BBa_K245008) have a ccdB gene inserted in their multiple cloning site. CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site as the ones listed above, cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. This part comprises only active ccdB part without inactive ccdA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216