Difference between revisions of "Part:BBa M36776:Experience"
(→User Reviews) |
|||
| Line 40: | Line 40: | ||
(Gene ID from DNA 2.0: 133530) | (Gene ID from DNA 2.0: 133530) | ||
| + | ''' | ||
''' | ''' | ||
== Experiment Steps ==''' | == Experiment Steps ==''' | ||
| − | + | ''' | |
For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media. | For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media. | ||
Revision as of 20:56, 5 December 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36776
User Reviews
UNIQ375e481eaa6272c7-partinfo-00000000-QINU UNIQ375e481eaa6272c7-partinfo-00000001-QINU
== Stanford Location ==
All glycerol stocks are made out of E. Coli K12 strain and stocked in the BioE44 F13 box. They are made for the testing of our pH sensor.
Matt's Glycerol Stock of the mutant from DNA 2.0: 0133009642
Sylvia's Glycerol Stock of the mutant: 0133009642
(Order ID from DNA 2.0 for the mutant sequence: 135227.)
(These two samples serve as replicant groups for the mutant samples for both out first and second tests.)
Glycerol stock of negative control (K12 cell from the teaching lab) : 0133011408
Glycerol stock of the positive control (E.coli with p82 plasmid made before): 0133010301
Glycerol stock of the pH sensor sequence without mutation: 0133010286
(Gene ID from DNA 2.0: 133530)
== Experiment Steps ==
For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media.