Difference between revisions of "Part:BBa M36776:Experience"

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'''== Stanford Location =='''
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All glycerol stocks are made out of E. Coli K12 strain and stocked in the BioE44 F13 box. They are made for the testing of our pH sensor.
  
 
Matt's Glycerol Stock of the mutant from DNA 2.0: 0133009642
 
Matt's Glycerol Stock of the mutant from DNA 2.0: 0133009642
  
 
Sylvia's Glycerol Stock of the mutant: 0133009642
 
Sylvia's Glycerol Stock of the mutant: 0133009642
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(Order ID from DNA 2.0 for the mutant sequence: 135227.)
  
 
(These two samples serve as replicant groups for the mutant samples for both out first and second tests.)
 
(These two samples serve as replicant groups for the mutant samples for both out first and second tests.)
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Glycerol stock of the positive control (E.coli with p82 plasmid made before): 0133010301
 
Glycerol stock of the positive control (E.coli with p82 plasmid made before): 0133010301
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Glycerol stock of the pH sensor sequence without mutation: 0133010286
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(Gene ID from DNA 2.0: 133530)
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'''
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== Experiment Steps =='''
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For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media.
 
For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media.

Revision as of 20:55, 5 December 2013

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== Stanford Location ==

All glycerol stocks are made out of E. Coli K12 strain and stocked in the BioE44 F13 box. They are made for the testing of our pH sensor.

Matt's Glycerol Stock of the mutant from DNA 2.0: 0133009642

Sylvia's Glycerol Stock of the mutant: 0133009642

(Order ID from DNA 2.0 for the mutant sequence: 135227.)

(These two samples serve as replicant groups for the mutant samples for both out first and second tests.)

Glycerol stock of negative control (K12 cell from the teaching lab) : 0133011408

Glycerol stock of the positive control (E.coli with p82 plasmid made before): 0133010301

Glycerol stock of the pH sensor sequence without mutation: 0133010286

(Gene ID from DNA 2.0: 133530)

== Experiment Steps ==


For the first round of testing, we did Beta-gel and GFP measurement at 2 hours and 18 hours. The 2 hour one didn't show significant sign of expression. Therefore, for the second round, we would test the expression 24 hours and 48 hours after we put them in media.