Difference between revisions of "Part:BBa K1132034"
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| + | '''''DO NOT USE this part! The FimE sites did not work well, this design can be use by replace K137008 by K1077001 and K137010 by K1077000.''''' | ||
This BioBrick is designed to test the AND gate ([https://parts.igem.org/Part:BBa_K1132003 BBa_K1132003]) by measuring the level of RFP after recombination events. The biobrick [https://parts.igem.org/Part:BBa_K081014 BBa_K081014] containing the RBS site, the coding sequence of the RFP has been inserted inside our gate between the FimE restrictions sites. However, to detect expression of RFP, the T7 polymerase is required. An amelioration of this BioBrick has been created, adding a strong promoter, an RBS and the open reading frame of the T7 polymerase to the construct ([https://parts.igem.org/Part:BBa_K1132037 BBa_K1132037]).<br><br> | This BioBrick is designed to test the AND gate ([https://parts.igem.org/Part:BBa_K1132003 BBa_K1132003]) by measuring the level of RFP after recombination events. The biobrick [https://parts.igem.org/Part:BBa_K081014 BBa_K081014] containing the RBS site, the coding sequence of the RFP has been inserted inside our gate between the FimE restrictions sites. However, to detect expression of RFP, the T7 polymerase is required. An amelioration of this BioBrick has been created, adding a strong promoter, an RBS and the open reading frame of the T7 polymerase to the construct ([https://parts.igem.org/Part:BBa_K1132037 BBa_K1132037]).<br><br> | ||
Latest revision as of 10:07, 14 November 2013
AND gate (BBa_K1132003) with RFP
DO NOT USE this part! The FimE sites did not work well, this design can be use by replace K137008 by K1077001 and K137010 by K1077000.
This BioBrick is designed to test the AND gate (BBa_K1132003) by measuring the level of RFP after recombination events. The biobrick BBa_K081014 containing the RBS site, the coding sequence of the RFP has been inserted inside our gate between the FimE restrictions sites. However, to detect expression of RFP, the T7 polymerase is required. An amelioration of this BioBrick has been created, adding a strong promoter, an RBS and the open reading frame of the T7 polymerase to the construct (BBa_K1132037).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 957
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 264
Illegal AgeI site found at 376 - 1000COMPATIBLE WITH RFC[1000]
See BBa_K1132037 for characterization.