Difference between revisions of "Part:BBa K1189030:Design"
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| + | <h1>Design notes</h1> | ||
| + | <p>The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.</p> | ||
| − | + | <h1>Source</h1> | |
| + | <p>The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.</p> | ||
| − | + | <h1>References</h1> | |
| + | <li>Litowski, J. R., & Hodges, R. S. (2002). Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity. <i>The Journal of Biological Chemistry, 277</i>(40), 37272–9. doi:10.1074/jbc.M204257200</li> | ||
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Latest revision as of 07:46, 2 November 2013
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design notes
The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.
Source
The TALE sequence was codon optimzed, a Kozak sequence eliminated, generated through a commericial synthesis company.