Difference between revisions of "Part:BBa K1189021"
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<partinfo>BBa_K1189021 short</partinfo>
 
 
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This part was created by the fusion of the heavy and light ferritin subunits (<partinfo>BBa_K1189025</partinfo>, <partinfo>BBa_K1189024</partinfo>) to form the fusion ferritin subunit. The lacI promoter (<partinfo>BBa_J04500</partinfo>), double terminator (<partinfo>BBa_B0015</partinfo>) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to <partinfo>BBa_K1189018</partinfo>, but instead of the E-coil, it is replaced by TALA(<partinfo>BBa_K1189004</partinfo>). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.
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<h1>Fusion of ferrtin subunits linked to DNA detectors</h1>
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The DNA-binding protein, TALA, found on this construct binds the shiga-toxin gene found in pathogenic bacteria, such as <i>E. coli</i>
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<p>Ferritin is a protein shelled nanoparticle and is composed of a mixture of 24 <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189024">light (BBa_K1189024)</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189025">heavy (BBa_K1189025)</a> subunits. It is ubiquitous across eukaryotic and prokaryotic systems and is used to sequester intracellular iron (Chasteen <i>et al.</i>, 1991). The <a href="http://2013.igem.org/Team:Calgary">2013 iGEM Calgary</a> used ferritin’s iron core as a reporter and its protein shell to scaffold <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Detector">DNA sensing TALEs</a> as part of their project, the <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor">FerriTALE</a> (see Figure 1).</p>
 
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<img src="https://static.igem.org/mediawiki/parts/0/0e/L-Ferritinh-FerritinHis-TALE_APlacI.png" width="250" height="100">
 
<img src="https://static.igem.org/mediawiki/parts/0/0e/L-Ferritinh-FerritinHis-TALE_APlacI.png" width="250" height="100">

Revision as of 02:27, 1 November 2013

Fusion of ferrtin subunits linked to DNA detectors

Ferritin is a protein shelled nanoparticle and is composed of a mixture of 24 light (BBa_K1189024) and heavy (BBa_K1189025) subunits. It is ubiquitous across eukaryotic and prokaryotic systems and is used to sequester intracellular iron (Chasteen et al., 1991). The 2013 iGEM Calgary used ferritin’s iron core as a reporter and its protein shell to scaffold DNA sensing TALEs as part of their project, the FerriTALE (see Figure 1).

Calgary2013-TALE-RVD.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3656