Difference between revisions of "Part:BBa K1189037:Design"
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===Design Notes===
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<html>
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DUNNO
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<a href="http://www.uniprot.org/uniprot/P02792">P02792 [UniParc]</a>
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<a href="http://www.uniprot.org/uniprot/P02794">P02794 [UniParc]</a>
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===Source===
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<h1>Design notes</h1>
  
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DUNNO
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<p>This part was created by fusing the heavy chain (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189025">BBa_K1189025</a>, <a href="http://www.uniprot.org/uniprot/P02794">P02794 [UniParc]</a>) and light chains (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189024">BBa_K1189024</a>, <a href="http://www.uniprot.org/uniprot/P02792">P02792 [UniParc]</a>) of human ferritin together. It is expressed under the LacI promoter (<a href="https://parts.igem.org/Part:BBa_J04500">BBa_J04500</a>) and has a his-tag for protein purification. An E-coil (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189011">BBa_K1189011</a>) is included in order to allow binding of parts containing the respective K-coil (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189010">BBa_K1189010</a>). This particular part is expressed under a strong RBS and the LacI promoter. The iGEM Calgary 2013 team attempted <a href="https://parts.igem.org/Part:BBa_K1189037#pSB1C3 expression">expression and purification</a> of E coil ferritin di-subunit using this system. Additionally, they tested this same coding sequence under the T7 promoter in a <a href="https://parts.igem.org/Part:BBa_K1189037:Experience#User reviews">different vector</a>.</p>
  
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===References===
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<h1>Source</h1>
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<li>Zhang, W., Zhang, Y., Chen, Y., Li, S., Gu, N., Hu, S., Sun, Y., Chen, X., & Li, Q. (2013). Prussian blue modified ferritin as peroxidase mimetics and its applications in biological detection, <i>Journal of Nanoscience and Nanotechnology, 12</i>, 1–8. doi:10.1166/jnn.2012.6871
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<h1>References</h1>
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</html>

Revision as of 23:30, 31 October 2013

His tag + Human ferritin di-subunit with E coil under LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1307


Design notes

This part was created by fusing the heavy chain (BBa_K1189025, P02794 [UniParc]) and light chains (BBa_K1189024, P02792 [UniParc]) of human ferritin together. It is expressed under the LacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010). This particular part is expressed under a strong RBS and the LacI promoter. The iGEM Calgary 2013 team attempted expression and purification of E coil ferritin di-subunit using this system. Additionally, they tested this same coding sequence under the T7 promoter in a different vector.

Source

References