Difference between revisions of "Part:BBa K1033918"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1033918 short</partinfo> | <partinfo>BBa_K1033918 short</partinfo> | ||
− | This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed. | + | This chromoprotein from the coral ''Galaxea fascicularis'', gfasPurple (also known as gfasCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 577 nm giving it a purple color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein gfasPurple is closely related to amilCP (<partinfo>BBa_K592009</partinfo>) and has significant sequence homologies with proteins in the GFP family. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part is useful as a reporter. | ||
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+ | ===Source=== | ||
+ | ''Galaxea fascicularis''. The protein was first extracted and characterized by Alieva et. al. under the name gfasCP (GenBank: DQ206394.1). This version is codon optimized for '' E coli'' by Genscript. | ||
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+ | ===References=== | ||
+ | [http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, Naila O., et al. "Diversity and evolution of coral fluorescent proteins." PLoS One 3.7 (2008): e2680. | ||
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Revision as of 19:26, 31 October 2013
gfasPurple, purple chromoprotein (incl RBS)
This chromoprotein from the coral Galaxea fascicularis, gfasPurple (also known as gfasCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 577 nm giving it a purple color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein gfasPurple is closely related to amilCP (BBa_K592009) and has significant sequence homologies with proteins in the GFP family.
Usage and Biology
This part is useful as a reporter.
Source
Galaxea fascicularis. The protein was first extracted and characterized by Alieva et. al. under the name gfasCP (GenBank: DQ206394.1). This version is codon optimized for E coli by Genscript.
References
[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, Naila O., et al. "Diversity and evolution of coral fluorescent proteins." PLoS One 3.7 (2008): e2680.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]