Difference between revisions of "Part:BBa K1104102:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1104102 short</partinfo>
 
<partinfo>BBa_K1104102 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
No
+
1. During blunt-end ligation, we should add a kinase, in order to phosphoylate the two ends of the DNA that we cloned. The ligase can only work when the DNA is phosphorylated.  <br>
−
 
+
2. After ligation, it is a must to do a PCR check to see if you have ligated the coding sequence in the right direction.
  
  
 
===Source===
 
===Source===
−
 
+
This part is ligated from different sources:
−
E.coli
+
1. Escherichia coli K-12 substrain MG1655 genomic sequence  <br>
−
 
+
2. Biobrick sent from the headquarter
 
===References===
 
===References===
 +
1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism  [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH] <br>
 +
2. Wellens, A., Lahmann, M., Touaibia, M., Vaucher, J., Oscarson, S., Roy, R., Remaut, H., ... Bouckaert, J. (January 01, 2012). The tyrosine gate as a potential entropic lever in the receptor-binding site of the bacterial adhesin FimH. Biochemistry, 51, 24, 4790-9.  <br>

Revision as of 21:09, 29 October 2013

pLac+RFP-FimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. During blunt-end ligation, we should add a kinase, in order to phosphoylate the two ends of the DNA that we cloned. The ligase can only work when the DNA is phosphorylated.
2. After ligation, it is a must to do a PCR check to see if you have ligated the coding sequence in the right direction.


Source

This part is ligated from different sources: 1. Escherichia coli K-12 substrain MG1655 genomic sequence
2. Biobrick sent from the headquarter

References

1. EcoCyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10315-MONOMER#/ FimH]
2. Wellens, A., Lahmann, M., Touaibia, M., Vaucher, J., Oscarson, S., Roy, R., Remaut, H., ... Bouckaert, J. (January 01, 2012). The tyrosine gate as a potential entropic lever in the receptor-binding site of the bacterial adhesin FimH. Biochemistry, 51, 24, 4790-9.