Difference between revisions of "Part:BBa K1021021"
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The gene for monomeric red fluorescent protein sits downstream of the T7 promoter for expression with T7 RNA Polymerase. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation. | The gene for monomeric red fluorescent protein sits downstream of the T7 promoter for expression with T7 RNA Polymerase. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | *This part was constructed from BBa_I712074 and BBa_E1010. | ||
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Latest revision as of 03:51, 29 October 2013
PT7+mRFP
The gene for monomeric red fluorescent protein sits downstream of the T7 promoter for expression with T7 RNA Polymerase. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation.
Usage and Biology
- This part was constructed from BBa_I712074 and BBa_E1010.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 607
Illegal AgeI site found at 719 - 1000COMPATIBLE WITH RFC[1000]