Difference between revisions of "Part:BBa K1021033"
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===Usage and Biology=== | ===Usage and Biology=== | ||
*The part was constructed from BBa_I712074 and BBa_K1021002. | *The part was constructed from BBa_I712074 and BBa_K1021002. | ||
| + | *Cornell iGEM characterized this part in <i>E. coli</i> BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005). | ||
| + | [[File:Hph.png]] | ||
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Latest revision as of 03:48, 29 October 2013
PT7+hph
The hygromycin phosphotransferase gene (hph), which also confers resistance to hygromycin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.
Usage and Biology
- The part was constructed from BBa_I712074 and BBa_K1021002.
- Cornell iGEM characterized this part in E. coli BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 391
- 1000COMPATIBLE WITH RFC[1000]