Difference between revisions of "Part:BBa K1021007"
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<partinfo>BBa_K1021007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1021007 SequenceAndFeatures</partinfo> | ||
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| + | To test the activity of the trpC promoter, a composite part was created with GFP downstream (BBa_K1021023). This part was transformed into the ascomycete fungi <i>Cochliobolus heterostrophus</i>. Results indicated that the trpC promoter provided GFP expression within this fungal chassis. | ||
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| + | https://static.igem.org/mediawiki/2013/6/60/Cochliobolus_fluorescence_composite.png | ||
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Revision as of 03:47, 29 October 2013
PtrpC
PtrpC is a strong constitutive promoter from Aspergillus nidulans's tryptophan biosynthesis pathways. It was isolated using a fungal transformation vector and constructed into the pSB1C3 backbone for continuous homologous and heterologous gene expression in fungal chassis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
To test the activity of the trpC promoter, a composite part was created with GFP downstream (BBa_K1021023). This part was transformed into the ascomycete fungi Cochliobolus heterostrophus. Results indicated that the trpC promoter provided GFP expression within this fungal chassis.
