Difference between revisions of "Part:BBa K1021009"
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| − | PpelA is an inducible pectate lyase promoter from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled gene expression can be achieved by cloning the desired gene downstream of PpelA. PpelA was constructed by digesting the | + | PpelA is an inducible pectate lyase promoter isolated from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled homologous and heterologous gene expression can be achieved by cloning the desired gene downstream of PpelA and adding glucose or polygalacturonic acid to the fungal culture. PpelA was constructed by digesting the fungal transformation vector pHNU3pelA with BamHI and KpnI restriction enzymes and subsequently ligating the digested fragment into a pSB1C3 backbone appended with BamHI and KpnI restriction sites. |
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Latest revision as of 03:28, 29 October 2013
PpelA
PpelA is an inducible pectate lyase promoter isolated from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled homologous and heterologous gene expression can be achieved by cloning the desired gene downstream of PpelA and adding glucose or polygalacturonic acid to the fungal culture. PpelA was constructed by digesting the fungal transformation vector pHNU3pelA with BamHI and KpnI restriction enzymes and subsequently ligating the digested fragment into a pSB1C3 backbone appended with BamHI and KpnI restriction sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]