Difference between revisions of "Part:BBa K1119011:Design"
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===Source=== | ===Source=== | ||
The EF-1alpha promoter ([[Part:BBa_K1119010|BBa_K1119010]]) sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/browse/sequence_vdb/2491/) using PCR with primers that includes prefix and suffix in RFC10 standard. | The EF-1alpha promoter ([[Part:BBa_K1119010|BBa_K1119010]]) sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/browse/sequence_vdb/2491/) using PCR with primers that includes prefix and suffix in RFC10 standard. | ||
− | GFP reporter in RFC25 format from partsregistry([[Part:BBa_K648013|BBa_K648013]]) | + | <p>GFP reporter in RFC25 format from partsregistry([[Part:BBa_K648013|BBa_K648013]])</p> |
− | hGH polyA terminator in RFC10 format from partsregistry ([[Part:BBa_K404108|BBa_K404108]]) | + | <p> hGH polyA terminator in RFC10 format from partsregistry ([[Part:BBa_K404108|BBa_K404108]]) </p> |
===References=== | ===References=== |
Revision as of 03:02, 29 October 2013
Human Elongation Factor-1alpha Promoter - GFP - hGH polyA tail
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 326
Illegal AgeI site found at 83
Illegal AgeI site found at 1067 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1470
Illegal BsaI.rc site found at 972
Design Notes
There are NgoMIV & AgeI sites around GFP reporter (BBa_K648013), which make the part incompatible with RFC25 standard as shown by Part Registry, but users can perform in-frame protein fusion using the mentioned sites.
Source
The EF-1alpha promoter (BBa_K1119010) sequence was cloned out from plasmid iDUET101a (http://www.addgene.org/browse/sequence_vdb/2491/) using PCR with primers that includes prefix and suffix in RFC10 standard.
GFP reporter in RFC25 format from partsregistry(BBa_K648013)
hGH polyA terminator in RFC10 format from partsregistry (BBa_K404108)