Difference between revisions of "Part:BBa K1119011:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | There are NgoMIV & AgeI sites around GFP reporter ([[Part:BBa_K648013|BBa_K648013]]), which make the part incompatible with RFC25 standard as shown by Part Registry, but users can perform in-frame protein fusion using the mentioned sites. | |
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===Source=== | ===Source=== |
Revision as of 02:58, 29 October 2013
Human Elongation Factor-1alpha Promoter - GFP - hGH polyA tail
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 326
Illegal AgeI site found at 83
Illegal AgeI site found at 1067 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1470
Illegal BsaI.rc site found at 972
Design Notes
There are NgoMIV & AgeI sites around GFP reporter (BBa_K648013), which make the part incompatible with RFC25 standard as shown by Part Registry, but users can perform in-frame protein fusion using the mentioned sites.
Source
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