Difference between revisions of "Part:BBa K1129005"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1129005 short</partinfo>
 
<partinfo>BBa_K1129005 short</partinfo>
−
 
−
ATCCR1 is a Cinnamoyl CoA reductase found in Artabidopsis thaliana it functions to reduce the cinnamoyl coA molecule into cinnamaldehyde by utilizing the favourable kinetics offered by releasing the coA group. This enzyme belongs to the oxioreductase family and is involved in both the phenylpropanoid pathway and thus in the degradation of lignin.
 
−
 
−
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 8:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1129005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1129005 SequenceAndFeatures</partinfo>
−
 
  
  
Line 19: Line 14:
 
<partinfo>BBa_K1129005 parameters</partinfo>
 
<partinfo>BBa_K1129005 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Background===
 +
----
 +
ATCCR1 is a Cinnamoyl CoA reductase found in Artabidopsis thaliana it functions to reduce the cinnamoyl coA molecule into cinnamaldehyde by utilizing the favourable kinetics offered by releasing the coA group. This enzyme belongs to the oxioreductase family and is involved in both the phenylpropanoid pathway and thus in the degradation of lignin.
 +
 +
 +
  
 
===DATA===
 
===DATA===

Revision as of 02:12, 29 October 2013

Cinnamoyl-CoA reductase from Arabidopsis thaliana

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 201
    Illegal XhoI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

ATCCR1 is a Cinnamoyl CoA reductase found in Artabidopsis thaliana it functions to reduce the cinnamoyl coA molecule into cinnamaldehyde by utilizing the favourable kinetics offered by releasing the coA group. This enzyme belongs to the oxioreductase family and is involved in both the phenylpropanoid pathway and thus in the degradation of lignin.



DATA


Const._EncP_%2B_AtCCR1.jpg

Figure 1. Compound generation identification by GC-MS. Chromatograms (left) and mass spectra (right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. Top) GC-MS of a Cinnamic acid (10.580) control. Bottom) Conversion of Phenylalanine to Cinnamaldehyde (11.819) through the cinnamic acid intermediate(10.580) via the EncP, 4CL, and ATCCR1 gene construct under a constitutive promoter. The mass spec data shows a highly oxidized cinnamaldehyde that may have resulted from poor sample preparation

Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://www.arabidopsis.org/servlets/TairObject?id=137093&type=locus

http://www.genome.jp/kegg-bin/show_pathway?map00940