Difference between revisions of "Part:BBa K1189034"

Revision as of 01:29, 29 October 2013

J04500_GFP_freiburg This is a the standard GFP reporter (E0040) that contains the prefix/suffix required for RFC25 standard assembly (K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 870

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 <partinfo>BBa_K1189034 short</partinfo>
 <html>
-This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for RFC 25 standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007).</html>
+This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for <a href="https://parts.igem.org/Help:Standards/Assembly/RFC25">RFC25</a> standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007).</html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===