Difference between revisions of "Part:BBa K1189034"
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<partinfo>BBa_K1189034 short</partinfo> | <partinfo>BBa_K1189034 short</partinfo> | ||
| − | i | + | This is a the standard GFP reporter (E0400) that contains the prefix/suffix required for RFC 25 standard assembly (BBa_K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller <i>et al,</i>, 2007). |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:15, 29 October 2013
J04500_GFP_freiburg
This is a the standard GFP reporter (E0400) that contains the prefix/suffix required for RFC 25 standard assembly (BBa_K648013) placed behind an IPTG inducible promoter (J04500). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007). Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870