Difference between revisions of "Part:BBa K1021032"

 
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<partinfo>BBa_K1021032 short</partinfo>
 
<partinfo>BBa_K1021032 short</partinfo>
  
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The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits behind the T7 promoter.
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The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.  
  
 
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Revision as of 00:27, 29 October 2013

PT7+nptII

The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 530
    Illegal SapI.rc site found at 740