Difference between revisions of "Part:BBa K1210001"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1210001 short</partinfo>
 
<partinfo>BBa_K1210001 short</partinfo>
  
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Synthetic pseudoknot that induces -1 frameshift during translation.
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Synthetic pseudoknot that induces -1 frameshift during translation. Part has flanking RFC 25 prefix and suffix upstream and downstream.
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RFC 25 prefix 5' GAATTCGCGGCCGCTTCTAGATGGCCGGC 3'
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RFC 25 suffix 5' ACCGGTTAATACTAGTAGCGGCCGCTGCAG 3'
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Assembly with this part should be done using the RFC 25 assembly strategy (which creates the following scar: 5' [part A] ACCGGC [part B] 3'). Assembling using RFC 23 (Silver standard) creates a stop codon in the -1 frame of the scar sequence (5' [part A] ACTAGA [part B] 3').
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:56, 28 October 2013

PK401 Pseudoknot

Synthetic pseudoknot that induces -1 frameshift during translation. Part has flanking RFC 25 prefix and suffix upstream and downstream.

RFC 25 prefix 5' GAATTCGCGGCCGCTTCTAGATGGCCGGC 3'

RFC 25 suffix 5' ACCGGTTAATACTAGTAGCGGCCGCTGCAG 3'

Assembly with this part should be done using the RFC 25 assembly strategy (which creates the following scar: 5' [part A] ACCGGC [part B] 3'). Assembling using RFC 23 (Silver standard) creates a stop codon in the -1 frame of the scar sequence (5' [part A] ACTAGA [part B] 3').


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]