Difference between revisions of "Part:BBa K1045002"

 
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This part is a reporter system.<br />  
 
This part is a reporter system.<br />  
It is composed of a riboswitch from ''Bacillus subtilis'' genomic DNA with its native promoter and its native RBS ([[Part:BBa_K1045004|BBa_K1045004]]), controlling the expression of ''cfp'' as a reporter gene ([[Part:BBa_E0020|BBa_E0020]]).
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It is composed of the ''ydaO'' riboswitch from ''Bacillus subtilis'' genomic DNA with its native promoter and its native RBS ([[Part:BBa_K1045004|BBa_K1045004]]), controlling the expression of ''cfp'' as a reporter gene ([[Part:BBa_E0020|BBa_E0020]]).
  
 
Ideally, in the presence of c-di-AMP, the riboswitch leads to premature termination of ''cfp'' transcription. In the absence of c-di-AMP, the system uses the terminator present in the '''pSB1C3''' backbone. This results in the formation of a ''cfp'' mRNA which can be translated in a functional CFP protein. Thus, in the abscence of c-di-AMP, the system should fluoresce. In the presence of c-di-AMP, however, the system should not fluoresce.
 
Ideally, in the presence of c-di-AMP, the riboswitch leads to premature termination of ''cfp'' transcription. In the absence of c-di-AMP, the system uses the terminator present in the '''pSB1C3''' backbone. This results in the formation of a ''cfp'' mRNA which can be translated in a functional CFP protein. Thus, in the abscence of c-di-AMP, the system should fluoresce. In the presence of c-di-AMP, however, the system should not fluoresce.

Latest revision as of 19:45, 28 October 2013

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

This part is a reporter system.
It is composed of the ydaO riboswitch from Bacillus subtilis genomic DNA with its native promoter and its native RBS (BBa_K1045004), controlling the expression of cfp as a reporter gene (BBa_E0020).

Ideally, in the presence of c-di-AMP, the riboswitch leads to premature termination of cfp transcription. In the absence of c-di-AMP, the system uses the terminator present in the pSB1C3 backbone. This results in the formation of a cfp mRNA which can be translated in a functional CFP protein. Thus, in the abscence of c-di-AMP, the system should fluoresce. In the presence of c-di-AMP, however, the system should not fluoresce.

Furthermore, in combination with the diadenylate cyclase domain (BBa_K1045003), the riboswitch reporter system could indicate the state of the c-di-AMP biosynthesis. This could be exploited in order to screen substances for effects on c-di-AMP homeostasis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]