Difference between revisions of "Part:BBa K737024:Experience"

Revision as of 16:05, 28 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K737024

User Reviews

UNIQ0b10251cd9dd55e5-partinfo-00000000-QINU UNIQ0b10251cd9dd55e5-partinfo-00000001-QINU

•••••

Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139200)

Fig. 1. Our phoA promoter assay result

Fig. 2. OUC-China 2012’s phoB promoter assay result (converted to bar chart)

Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).

@@ Line 29: / Line 29: @@
 <b>1. Sequence</b><br>
 No NotI restriction site in prefix and suffix. <br>
-bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)<br>
+bp deletion and 2bp mutation exist between the promoter and RBS.<br>
 <b>2. Result of our experiment</b><br>
-We improved this OUC-China’s phosphate sensor part including <i>phoB</i> promoter by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br>
+We improved this OUC-China’s phosphate sensor part including the <i>phoB</i> promoter, by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br>
 [[Image:Titech2013_parts_K1139201_Fig4.jpg|thumb|left|500px|'''Fig. 1.''' Our <i>phoA</i> promoter assay result]]