Difference between revisions of "Part:BBa K1189033"
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<p>The <a href="2013.igem.org/Team:Calgary">Calgary iGEM 2013</a> team sought to detect pathogenic enterohemorrhagic <i>E. coli</i> (EHEC) DNA using pairs of TALEs designed to pathogenic <i>E. coli</i> markers. This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains. This TALE, along with the complementary <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189032">BBa_K1189032</a>, will eventually be integrated into the final <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Prototype">lateral flow strip FerriTALE prototype</a>.</p> | <p>The <a href="2013.igem.org/Team:Calgary">Calgary iGEM 2013</a> team sought to detect pathogenic enterohemorrhagic <i>E. coli</i> (EHEC) DNA using pairs of TALEs designed to pathogenic <i>E. coli</i> markers. This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains. This TALE, along with the complementary <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189032">BBa_K1189032</a>, will eventually be integrated into the final <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Prototype">lateral flow strip FerriTALE prototype</a>.</p> | ||
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| + | <p>Please see the <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Detector#Engineered TALEs">iGEM Calgary 2013 Wiki</a> for more details about how these TALEs were engineered for optimal binding.</a> | ||
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Revision as of 10:07, 28 October 2013
EHEC TALE2 (binds to a 19bp region of stx2 gene)
The Calgary iGEM 2013 team sought to detect pathogenic enterohemorrhagic E. coli (EHEC) DNA using pairs of TALEs designed to pathogenic E. coli markers. This TALE was designed based on a sequence alignment of the stx2 gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including E. coli O157:H7, as well as several other EHEC strains. This TALE, along with the complementary BBa_K1189032, will eventually be integrated into the final lateral flow strip FerriTALE prototype.
Please see the iGEM Calgary 2013 Wiki for more details about how these TALEs were engineered for optimal binding.
Figure 1. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.
The Calgary team has tested their DNA detection system using proof of concept TALEs designed by the 2012 Slovenia iGEM team. They have assembled BBa_K1189033 as shown in Figure 2 and are working on expressing, testing, and integrating these protein components into the final system.
Figure 2. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2404
Illegal BamHI site found at 2539 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]