Difference between revisions of "Part:BBa K1189033"
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<p>This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains.</p>
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<p>The Calgary iGEM 2013 team sought to detect pathogenic enterohemorrhagic <i>E. coli</i> (EHEC) DNA using pairs of TALEs designed to pathogenic <i>E. coli</i> markers. This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains.</p>
  
 
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<img src="https://static.igem.org/mediawiki/2013/b/ba/ET2_gel_finalSOEPCR.png" alt="EHEC TALE 2 SOE PCR Product" width="300" height="400">
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<img src="https://static.igem.org/mediawiki/2013/5/50/UCalgary2013TRTwotargetTALE.png" alt="EHEC TALE 2 SOE PCR Product" width="300" height="400">
 
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<p><b>Figure 1.</b> Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.</p>
 
<p><b>Figure 1.</b> Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.</p>
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<img src="https://static.igem.org/mediawiki/2013/b/ba/ET2_gel_finalSOEPCR.png" alt="EHEC TALE 2 SOE PCR Product" width="300" height="400">
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<p><b>Figure 2.</b> Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.</p>
 
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Revision as of 09:42, 28 October 2013

EHEC TALE2 (binds to a 19bp region of stx2 gene)

The Calgary iGEM 2013 team sought to detect pathogenic enterohemorrhagic E. coli (EHEC) DNA using pairs of TALEs designed to pathogenic E. coli markers. This TALE was designed based on a sequence alignment of the stx2 gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including E. coli O157:H7, as well as several other EHEC strains.

EHEC TALE 2 SOE PCR Product

Figure 1. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.

<figure> <img src="ET2_gel_finalSOEPCR.png" alt="EHEC TALE 2 SOE PCR Product" width="300" height="400"> <figcaption>

Figure 2. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.

</figcaption> </figure> </html>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2404
    Illegal BamHI site found at 2539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]