Difference between revisions of "Part:BBa K1189033"
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<p>This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains.</p>
 
<p>This TALE was designed based on a sequence alignment of the <i>stx2</i> gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including <i>E. coli O157:H7</i>, as well as several other EHEC strains.</p>
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<img src="https://static.igem.org/mediawiki/2013/b/ba/ET2_gel_finalSOEPCR.png" alt="EHEC TALE 2 SOE PCR Product" width="300" height="300">
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<p><b>Figure 1.</b> Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.</p>
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Revision as of 09:34, 28 October 2013

EHEC TALE2 (binds to a 19bp region of stx2 gene)

This TALE was designed based on a sequence alignment of the stx2 gene found in EHEC organisms. It binds to a 19 bp region of the toxin, will detect EHEC strains including E. coli O157:H7, as well as several other EHEC strains.

EHEC TALE 2 SOE PCR Product

Figure 1. Final size of the EHEC TALE 2 generated from IDT Gene Blocks using SOE PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2404
    Illegal BamHI site found at 2539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]