Difference between revisions of "Part:BBa K1149001"
 
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This part if a collection of secretion tags and fusion-partners for secretion.  
 
This part if a collection of secretion tags and fusion-partners for secretion.  
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All of these are designed for extracellular targeting of proteins in E.coli. You can learn about the different secretion strategies and the use of these at the secretion section of the 2013 Imperial College team wiki.  
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All of these are designed for extracellular targeting of proteins in E.coli. You can learn about the different secretion strategies and the use of these at the secretion section of the 2013 Imperial College team' [http://2013.igem.org/Team:Imperial_College/SecretionHelp Secretion Guide page].  
  
  

Latest revision as of 22:20, 27 October 2013

protein secretion in E.coli

This part if a collection of secretion tags and fusion-partners for secretion. All of these are designed for extracellular targeting of proteins in E.coli. You can learn about the different secretion strategies and the use of these at the secretion section of the 2013 Imperial College team' [http://2013.igem.org/Team:Imperial_College/SecretionHelp Secretion Guide page].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 122
    Illegal BamHI site found at 221
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 378
    Illegal SpeI site found at 1010
    Illegal NgoMIV site found at 1053
    Illegal NgoMIV site found at 2180
  • 1000
    COMPATIBLE WITH RFC[1000]