Difference between revisions of "Part:BBa K1139025"
 
Line 3: Line 3:
  
 
M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
 
M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
−
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin　and a packaging sequence. The promoter upstream of <i>g2p</i> is altered to <i>bad</i> promoter. A phage particle is formed only when the host cell receives arabinose because <i>g2p</i> (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.<br>
+
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin　and a packaging sequence. The promoter upstream of <i>g2p</i> (gene 2 protein) is altered to <i>bad</i> promoter. A phage particle is formed only when the host cell receives arabinose because <i>g2p</i> is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:52, 27 October 2013

Pbad-M13 on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin　and a packaging sequence. The promoter upstream of g2p (gene 2 protein) is altered to bad promoter. A phage particle is formed only when the host cell receives arabinose because g2p is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 136