Difference between revisions of "Part:BBa K1172588:Design"

 
 
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<partinfo>BBa_K1172588 short</partinfo>
 
<partinfo>BBa_K1172588 short</partinfo>
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===Design Notes===
 
===Design Notes===
  
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In order to combine the ''oprF'' BioBrick under control of the T7 promoter <bbpart>BBa_K1172502</bbpart> with the ''rib''-gene-cluster <bbpart>BBa_K1172303</bbpart> we used the [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Standard_BioBrick_Assembly Standard BioBrick Assembly Suffix Insertion].
  
  

Latest revision as of 19:47, 27 October 2013

Combination of oprF and rib-synth coding sequence under control of T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 802
    Illegal XhoI site found at 2456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1194
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 715


Design Notes

In order to combine the oprF BioBrick under control of the T7 promoter BBa_K1172502 with the rib-gene-cluster BBa_K1172303 we used the [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Standard_BioBrick_Assembly Standard BioBrick Assembly Suffix Insertion].



Source

References