Difference between revisions of "Part:BBa K1172599:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1172599 short</partinfo> | <partinfo>BBa_K1172599 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
+ | In order to combine the ''oprF'' BioBrick <bbpart>BBa_K1172501</bbpart> with the ''rib''-gene-cluster <bbpart>BBa_K1172303</bbpart> we used the [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Standard_BioBrick_Assembly Standard BioBrick Assembly Suffix Insertion]. | ||
Latest revision as of 19:45, 27 October 2013
Combination of oprF and rib-synth coding sequences
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 764
Illegal XhoI site found at 2418 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1156
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 677
Design Notes
In order to combine the oprF BioBrick BBa_K1172501 with the rib-gene-cluster BBa_K1172303 we used the [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Standard_BioBrick_Assembly Standard BioBrick Assembly Suffix Insertion].