Difference between revisions of "Part:BBa K1088015"
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<partinfo>BBa_K1088015 short</partinfo>
 
<partinfo>BBa_K1088015 short</partinfo>
  
−
This parts is the same as BBa_K1088013, but with the IspG gene translationally coupled to it (seperated by a scarsite).
+
This parts is the same as BBa_K1088013, but with the IspG gene (BBa_K1088004) translationally coupled to it seperated by a scarsite.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:57, 26 October 2013

IPTG controlled B. sub. dxs coupled with IspG from E. coli

This parts is the same as BBa_K1088013, but with the IspG gene (BBa_K1088004) translationally coupled to it seperated by a scarsite.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3162
    Illegal PstI site found at 2563
    Illegal PstI site found at 3009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3162
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2563
    Illegal PstI site found at 3009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3162
    Illegal BglII site found at 3779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3162
    Illegal PstI site found at 2563
    Illegal PstI site found at 3009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3162
    Illegal PstI site found at 2563
    Illegal PstI site found at 3009
    Illegal NgoMIV site found at 2462
    Illegal AgeI site found at 2355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2304
    Illegal SapI.rc site found at 3003
    Illegal SapI.rc site found at 3693