Difference between revisions of "Part:BBa J61015"
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The registry sequence is blunt relative to XbaI/SpeI. | The registry sequence is blunt relative to XbaI/SpeI. | ||
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Latest revision as of 22:41, 11 August 2006
Biobrick converter for NotI-part-BamHI
Inserting a NotI/BamHI fragment into the PspOMI/BglII sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.
The registry sequence is blunt relative to XbaI/SpeI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 930
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 641
Illegal AgeI site found at 753 - 1000COMPATIBLE WITH RFC[1000]