Difference between revisions of "Part:BBa K1031019"

Revision as of 06:13, 26 October 2013

J23114-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter

Characteristics

K1031019 is designed to test the activation effect of φR73Δ to hybrid promoter. It is composed of NahR, Psal promoter which is activated by NahR when exposed to its' inducer salicylic acid, RBS B0034, RiboJ and φR73Δ.

Fig 1 Construction of activation circuit of Band-pass Filter. The combination of φR73Δ with hybrid promoter and sfGFP could test if φR73Δ really works as an activator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
1000
COMPATIBLE WITH RFC[1000]

Data shown

We exposed this activation circuit to the typical inducer of NahR, salicylic acid, and controlled the expression level of φR73Δ via tuning the inducer's concentration. The activation effect of φR73Δ to hybrid promoter is shown as fluorescence intensity.

Fig 2 Effect of activation circuit of Band-pass Filter. Horizontal axis stands for different concentration of NahR inducer, salicylic acid. Vertical axis represents fluorescence intensity. From Fig 2 it is obvious that φR74Δ indeed activates the hybrid promoter, thus initiates the expression of sfGFP.

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 <p style="text-align:center"><b>Fig 1</b> Construction of activation circuit of Band-pass Filter. The combination of φR73Δ with hybrid promoter and sfGFP could test if φR73Δ really works as an activator.
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