Difference between revisions of "Part:BBa K1189021"
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This part was created by the fusion of the heavy and light ferritin subunits (<partinfo>BBa_K1189025</partinfo>, <partinfo>BBa_K1189024</partinfo>) to form the fusion ferritin subunit. The lacI promoter (<partinfo>BBa_J04500</partinfo>), double terminator (<partinfo>BBa_B0015</partinfo>) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to <partinfo>BBa_K1189018</partinfo>, but instead of the E-coil, it is replaced by TALA(<partinfo>BBa_K1189004</partinfo>). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.
 
This part was created by the fusion of the heavy and light ferritin subunits (<partinfo>BBa_K1189025</partinfo>, <partinfo>BBa_K1189024</partinfo>) to form the fusion ferritin subunit. The lacI promoter (<partinfo>BBa_J04500</partinfo>), double terminator (<partinfo>BBa_B0015</partinfo>) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to <partinfo>BBa_K1189018</partinfo>, but instead of the E-coil, it is replaced by TALA(<partinfo>BBa_K1189004</partinfo>). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.
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The DNA-binding protein, TALA, found on this construct binds the shiga-toxin gene found in pathogenic bacteria, such as <i>E.coli</i>
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The DNA-binding protein, TALA, found on this construct binds the shiga-toxin gene found in pathogenic bacteria, such as <i>E. coli</i>
 
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Revision as of 00:51, 26 October 2013

TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

This part was created by the fusion of the heavy and light ferritin subunits (BBa_K1189025, BBa_K1189024) to form the fusion ferritin subunit. The lacI promoter (BBa_J04500), double terminator (BBa_B0015) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to BBa_K1189018, but instead of the E-coil, it is replaced by TALA(BBa_K1189004). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold. The DNA-binding protein, TALA, found on this construct binds the shiga-toxin gene found in pathogenic bacteria, such as E. coli

Calgary2013-TALE-RVD.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3656