Difference between revisions of "Part:BBa M36661"
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Revision as of 21:11, 25 October 2013
E. coli fur protein
The fur protein is found in E. coli and is involved in iron regulation. The protein dimerizes in the presence of iron, thus inhibiting transcription. At the end of the fur protein, we included a his-tag to be used as the primary antibody binding site in a Western blot assay. The his-tag is also followed by a stop codon.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 298
Illegal PstI site found at 73 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 298
Illegal PstI site found at 73 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 298
Illegal BglII site found at 108 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 298
Illegal PstI site found at 73 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 298
Illegal PstI site found at 73 - 1000COMPATIBLE WITH RFC[1000]