Difference between revisions of "Part:BBa M36661"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M36661 short</partinfo>
 
<partinfo>BBa_M36661 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_M36661 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M36661 SequenceAndFeatures</partinfo>
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Fully annotated primary DNA/RNA sequence: (note mutations and changes made to original sequence)
 +
(start codon included in bicistronic design RBS:)ACTGACAACAACACTGCACTGAAAAAAGCTGGCCTGAAAGTAACCCTTCCTCGCCTGAAAATCCTGGAAGTGCTGCAGGAACCGGACAACCACCACGTCAGCGCAGAAGATCTGTACAAACGCCTGATCGACATGGGGGAGGAGATCGGTCTGGCAACCGTCTATCGTGTTTTGAATCAGTTTGACGACGCAGGTATCGTGACCCGCCACAACTTCGAAGGCGGTAAAAGCGTCTTTGAACTGACCCAACAGCACCACCACGATCACCTGATCTGCCTGGACTGCGGCAAAGTGATCGAATTCAGCGACGATAGTATTGAAGCACGTCAGCGTGAAATCGCTGCAAAGCACGGTATCCGTTTGACGAACCACAGCCTGTATCTTTACGGCCATTGTGCCGAAGGTGATTGCCGTGAAGATGAGC
  
  

Revision as of 21:07, 25 October 2013

E. coli fur protein

The fur protein is found in E. coli and is involved in iron regulation. The protein dimerizes in the presence of iron, thus inhibiting transcription. At the end of the fur protein, we included a his-tag to be used as the primary antibody binding site in a Western blot assay. The his-tag is also followed by a stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 108
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 1000
    COMPATIBLE WITH RFC[1000]

Fully annotated primary DNA/RNA sequence: (note mutations and changes made to original sequence) (start codon included in bicistronic design RBS:)ACTGACAACAACACTGCACTGAAAAAAGCTGGCCTGAAAGTAACCCTTCCTCGCCTGAAAATCCTGGAAGTGCTGCAGGAACCGGACAACCACCACGTCAGCGCAGAAGATCTGTACAAACGCCTGATCGACATGGGGGAGGAGATCGGTCTGGCAACCGTCTATCGTGTTTTGAATCAGTTTGACGACGCAGGTATCGTGACCCGCCACAACTTCGAAGGCGGTAAAAGCGTCTTTGAACTGACCCAACAGCACCACCACGATCACCTGATCTGCCTGGACTGCGGCAAAGTGATCGAATTCAGCGACGATAGTATTGAAGCACGTCAGCGTGAAATCGCTGCAAAGCACGGTATCCGTTTGACGAACCACAGCCTGTATCTTTACGGCCATTGTGCCGAAGGTGATTGCCGTGAAGATGAGC