Difference between revisions of "Part:BBa K1129001"

Revision as of 02:42, 25 October 2013

Feruloyl-CoA-Synthetase (FCS) from Pseudomonas putida KT2440

This is a feruloyl-CoA-synthetase (fcs) from Pseudomonas putida KT2440 which converts ferrulic acid to feruloyl CoA.

DATA

Figure 1 Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 43
Illegal XhoI site found at 1153
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 30
Illegal NgoMIV site found at 294
Illegal NgoMIV site found at 772
Illegal NgoMIV site found at 1027
Illegal NgoMIV site found at 1090
Illegal NgoMIV site found at 1107
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 802

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+===DATA===
+----
+<p align=center>https://static.igem.org/mediawiki/2013/a/ac/Ech900.jpg</p>
+'''Figure 1''' Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.