Difference between revisions of "Part:BBa K1172503:Design"

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(Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
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*Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Suffix_Insertion Suffix-Insertion] of the Biobrick <bbpart>BBa_K1172501</bbpart> into the Backbone of the BioBrick <bbpart>BBa_K04500</bbpart>.
 
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===Source===
 
===Source===

Latest revision as of 19:48, 22 October 2013


Outer membrane porin OprF from Pseudomonas fluorescens with Plac and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 990
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 903


Design Notes

  • Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Suffix_Insertion Suffix-Insertion] of the Biobrick BBa_K1172501 into the Backbone of the BioBrick BBa_K04500.

Source

Pseudomonas fluorescens Migula 1895 genomic DNA

DSMZ No.: 7355

References