Difference between revisions of "Part:BBa K1172201:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1172201 short</partinfo>
 
<partinfo>BBa_K1172201 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Primeroverlap for Gibson-Assembly
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'''Amplification with Gibson-primer:'''
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*Amplification of the glycerol dehydrogenase (''gldA''):<br>
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'''Forward primer gldA (43 bp)'''<br>
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5'-GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC-3'<br>
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'''Reverse primer gldA (45 bp)'''<br>
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5'-CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG-3'<br>
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*Amplification of the pSB1C3-Backbone:<br>
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'''Forward primer pSB1C3 gldA (43 bp)'''<br>
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5'-TCCTGCAAGAGTGGGAATAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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'''Reverse primer pSB1C3 gldA (43 bp)'''<br>
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5'-GATTGAATAATGCGGTCCATCTAGAAGCGGCCGCGAATTCCAG-3'<br>
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'''Ligation via [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Gibson_assembly Gibson-Assembly].'''
  
  
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===Source===
 
===Source===
  
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''Escherichicha coli'' KRX genomic sequence
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''Escherichicha coli'' KRX genomic sequence ([http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Whole_Genome_Isolation Whole Genome Isolation])
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===References===
 
===References===

Latest revision as of 18:12, 22 October 2013

GldA - glycerol dehydrogenase from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplification with Gibson-primer:

  • Amplification of the glycerol dehydrogenase (gldA):
Forward primer gldA (43 bp)

5'-GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC-3'

Reverse primer gldA (45 bp)

5'-CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG-3'
  • Amplification of the pSB1C3-Backbone:
Forward primer pSB1C3 gldA (43 bp)

5'-TCCTGCAAGAGTGGGAATAATACTAGTAGCGGCCGCTGCAGTC-3'

Reverse primer pSB1C3 gldA (43 bp)

5'-GATTGAATAATGCGGTCCATCTAGAAGCGGCCGCGAATTCCAG-3'


Ligation via [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Gibson_assembly Gibson-Assembly].


Source

Escherichicha coli KRX genomic sequence ([http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Whole_Genome_Isolation Whole Genome Isolation])


References