Difference between revisions of "Part:BBa K1045011"
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<partinfo>BBa_K1045011 short</partinfo><br/ > | <partinfo>BBa_K1045011 short</partinfo><br/ > | ||
| − | [[Part:BBa_K1045011|BBa_K1045011]] encodes for a weak promoter based on [[Part:BBa_J23117|BBa_J23117]] promoter from the well characterized Anderson promoter collection. Yet, the promoter in [[Part:BBa_K1045011|BBa_K1045011]] is inverted. We improved the inverted promoter [[Part:BBa_J23117|BBa_J23117]] by adding additional base pairs upstream of the inverted promoter region. These additional base pairs will allow cloning of this promoter directly in front of another promoter oriented in the opposite direction. This way, a vector with two different expression units oriented in diverging directions can be created. The extra bases prevent that the binding of the RNA polymerase to the usual promoter influences the binding of the RNA polymerase to the inverse promoter in [[Part:BBa_K1045011|BBa_K1045011]] (for an example see [[Part:BBa_K1045017|BBa_K1045017]], a | + | [[Part:BBa_K1045011|BBa_K1045011]] encodes for a weak promoter based on [[Part:BBa_J23117|BBa_J23117]] promoter from the well characterized Anderson promoter collection. Yet, the promoter in [[Part:BBa_K1045011|BBa_K1045011]] is inverted. We improved the inverted promoter [[Part:BBa_J23117|BBa_J23117]] by adding additional base pairs upstream of the inverted promoter region. These additional base pairs will allow cloning of this promoter directly in front of another promoter oriented in the opposite direction. This way, a vector with two different expression units oriented in diverging directions can be created. The extra bases prevent that the binding of the RNA polymerase to the usual promoter influences the binding of the RNA polymerase to the inverse promoter in [[Part:BBa_K1045011|BBa_K1045011]] (for an example see [[Part:BBa_K1045017|BBa_K1045017]], a detection system for c-di-AMP). |
'''Why this part is improved: ''' | '''Why this part is improved: ''' | ||
Revision as of 13:34, 19 October 2013
Promoter reverse
BBa_K1045011 encodes for a weak promoter based on BBa_J23117 promoter from the well characterized Anderson promoter collection. Yet, the promoter in BBa_K1045011 is inverted. We improved the inverted promoter BBa_J23117 by adding additional base pairs upstream of the inverted promoter region. These additional base pairs will allow cloning of this promoter directly in front of another promoter oriented in the opposite direction. This way, a vector with two different expression units oriented in diverging directions can be created. The extra bases prevent that the binding of the RNA polymerase to the usual promoter influences the binding of the RNA polymerase to the inverse promoter in BBa_K1045011 (for an example see BBa_K1045017, a detection system for c-di-AMP).
Why this part is improved:
1. BBa_J23117 was inverted (pre- and suffix switched).
2. BBa_K1045011 has additional bases in front of its promoter region to make it possible to clone it in front of a promoter oriented in the other direction.
3. Since the Sequence is just inverted between the pre- and suffix, this promoter can be used with other inverted biobricks (for example: BBa_K1045001).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NheI site found at 24 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
- 1000COMPATIBLE WITH RFC[1000]