Difference between revisions of "Part:BBa K1045001"
Line 3: Line 3:
  
 
This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]). <br/ >
 
This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from ''Mycobacterium smegmatis'', which can bind its specific binding sequence (the DarR operator [[Part:BBa_K1045000|BBa_K1045000]]). <br/ >
−
The binding effinciency is dependent on the availability of cyclic di-AMP, which enhances the probability of binding to DNA. Ideally, this protein will shut down transcription of information downstream of its operator. This way, transcription can be controlled via the level of c-di-AMP (e.g. for a reporter-system in order to monitor it).<br/ >
+
In ''M. smegmatis'', it was shown that the binding effinciency of DarR to the operator is enhanced by the availability of cyclic di-AMP. When bound to the operator, DarR shuts down transcription of the gene downstream of its operator. This way, transcription is controlled via c-di-AMP (Zhang ''et al''., 2013).<br/ >
  
  

Revision as of 12:47, 19 October 2013

DarR ORF with inversed Pre- and Suffix

This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR operator BBa_K1045000).
In M. smegmatis, it was shown that the binding effinciency of DarR to the operator is enhanced by the availability of cyclic di-AMP. When bound to the operator, DarR shuts down transcription of the gene downstream of its operator. This way, transcription is controlled via c-di-AMP (Zhang et al., 2013).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]