Difference between revisions of "Part:BBa K1045015:Design"
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===Source=== | ===Source=== | ||
| − | The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence information of the RBS originated from the Parts Registry page of [[Part:BBa_B0034|BBa_B0034]]. In addition, to construct the composite part '''BBa_K1045015''', [[Part:BBa_K1045014|BBa_K1045014]] was used. | + | The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence information of the RBS originated from the Parts Registry page of [[Part:BBa_B0034|BBa_B0034]]. In addition, to construct the composite part '''BBa_K1045015''', [[Part:BBa_K1045014|BBa_K1045014]] was used. [[Part:BBa_K1045014|BBa_K1045014]] was constructed using hybridization oligos (sequence information: Zhang et al., 2013 or parts registry) or parts taken from the distribution kit 2013. |
===References=== | ===References=== | ||
Revision as of 15:16, 16 October 2013
RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 21
Illegal NheI site found at 44
Illegal NheI site found at 124
Illegal NheI site found at 147 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 75
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 845
Design Notes
The inverse RBS was generated by hybridizing oligos to a double-stranded DNA fragment that corresponded to the inverse RBS sequence cut with EcoRI and SpeI at the prefix and suffix sequence. Next, this hybridization product was cloned in a prefixing composition into BBa_K1045014.
Source
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence information of the RBS originated from the Parts Registry page of BBa_B0034. In addition, to construct the composite part BBa_K1045015, BBa_K1045014 was used. BBa_K1045014 was constructed using hybridization oligos (sequence information: Zhang et al., 2013 or parts registry) or parts taken from the distribution kit 2013.