Difference between revisions of "Part:BBa K823039:Design"

(→‎Source)
 
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===Source===
 
===Source===
  
−
Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy] or maybe not... sorry this is going to be re-checked.
+
Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy]. In contrast to the reference below, the plasmid contains gfpmut1 (not gfpmut3).
  
 
===References===
 
===References===
 
[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)]
 
[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)]

Latest revision as of 12:22, 16 October 2013

gfpmut1 (B. subtilis optimized + Freiburg standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197


Design Notes

We designed this part in the Freiburg standard for N- and C-terminal fusions.


Source

Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy]. In contrast to the reference below, the plasmid contains gfpmut1 (not gfpmut3).

References

[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)]