Difference between revisions of "Part:BBa K1093017"

Revision as of 18:38, 12 October 2013

mCherry C-terminal

This is C-terminal of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). plit between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye. Alongside BBa_K1093016 this part can be used for investigating protein-protein interactions.

References

1: Fan JY, Cui ZQ, Wei HP, Zhang ZP, Zhou YF, Wang YP, Zhang XE. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochem Biophys Res Commun. 2008 Feb 29;367(1):47-53. Epub 2007 Dec 26. PubMed PMID: 18158915.

2: Kodama Y, Hu CD. Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. Biotechniques. 2012 Nov;53(5):285-98. doi: 10.2144/000113943. Review. PubMed PMID: 23148879.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1093017 short</partinfo>
-This is N-terminal of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). plit between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye. Alongside BBa_K1093016 this part can be used for investigating protein-protein interactions.
+This is C-terminal of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC). plit between amino acids 159 and 160. Emission/excitation maxima are 587/610 nm. Known to have short maturation time and can be potentially observed with naked eye. Alongside BBa_K1093016 this part can be used for investigating protein-protein interactions.
 ==References==