Difference between revisions of "Part:BBa K1055000:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| + | ===Applications of BBa_K1055001=== | ||
| + | When tried to fuse to the cheB signaling protein of ''E. coli'' C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 232 kj/mol). Please take a look below: | ||
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| + | [[Image:CMK_scstr.png|center|400px|thumb|Hypothetical DNA secondary structure of Tar-LssmOrange fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 318 kj/mol]] | ||
Revision as of 18:18, 12 October 2013
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how you used this part and how it worked out.
Applications of BBa_K1055001
When tried to fuse to the cheB signaling protein of E. coli C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 232 kj/mol). Please take a look below:
User Reviews
UNIQ65a94c0e6c411fb4-partinfo-00000000-QINU UNIQ65a94c0e6c411fb4-partinfo-00000001-QINU