Difference between revisions of "Part:BBa K1093003"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1093003 short</partinfo>
 
<partinfo>BBa_K1093003 short</partinfo>
  
 
Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm.  
 
Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm.  
−
This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC).
+
This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific).
 +
 
 +
==References==
 +
1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and
 +
characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006
 +
Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
 +
Sep;24(9):1170. PubMed PMID: 16369541.
 +
2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence
 +
complementation tool based on superfolder green fluorescent protein. Acta Biochim
 +
Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub
 +
2011 Jan 27. PubMed PMID: 21273204.
 +
 
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:16, 12 October 2013

sfBFP N-terminal

Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm. This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541. 2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub 2011 Jan 27. PubMed PMID: 21273204.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13