Difference between revisions of "Part:BBa K1071002"
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Chloramphenicol interacts with the 50s Subunit of bacterial ribosomes and blocks peptidyltransferase activity. The antibiotic is acting on gram negative as well as on gram positive bacteria. The enzyme Chloramphenicol acetyltransferase (CAT) transfers one or two Acetyl groups to the hydroxyl-Residues of Chloramphenicol. This inactivates the antibiotic. | Chloramphenicol interacts with the 50s Subunit of bacterial ribosomes and blocks peptidyltransferase activity. The antibiotic is acting on gram negative as well as on gram positive bacteria. The enzyme Chloramphenicol acetyltransferase (CAT) transfers one or two Acetyl groups to the hydroxyl-Residues of Chloramphenicol. This inactivates the antibiotic. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This chloramphenicol resistance gene was used in our expression vector to cultivate the plasmid in ''E. coli''. We successfully selected the colonies on LB plates with chloramphenicol. | ||
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Latest revision as of 18:05, 12 October 2013
cat resistance (procaryotic)
Chloramphenicol interacts with the 50s Subunit of bacterial ribosomes and blocks peptidyltransferase activity. The antibiotic is acting on gram negative as well as on gram positive bacteria. The enzyme Chloramphenicol acetyltransferase (CAT) transfers one or two Acetyl groups to the hydroxyl-Residues of Chloramphenicol. This inactivates the antibiotic.
Usage and Biology
This chloramphenicol resistance gene was used in our expression vector to cultivate the plasmid in E. coli. We successfully selected the colonies on LB plates with chloramphenicol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]