Difference between revisions of "Part:BBa K1093002"

Latest revision as of 18:03, 12 October 2013

sfCFP

Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence. Excitation peak: 425 nm Emission peak: 475 nm

Team Warsaw 2013 measured sfCFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

-	Samples			Arithmetic mean	Standard deviation
sfGFP	100,60	100,37	99,02	100,00	0,85
sfCFP	17,95	18,38	17,36	17,90	0,51
sfBFP	40,65	41,52	42,25	41,47	0,80
sfYFP	218,87	216,51	217,87	217,75	1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

Usage and Biology

sfCFP can be used as reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 46: / Line 46: @@
 |}
 ''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
+==References==
+: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and
+characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006
+Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
+Sep;24(9):1170. PubMed PMID: 16369541.
 ===Usage and Biology===