Difference between revisions of "Part:BBa K1071009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | In the following picture we demostrate that both promotors (i.e. <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1071004">PNR</a> | + | In the following picture we demostrate that both promotors (i.e. <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1071004">PNR (496)</a> </html> and <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1071003">PfcpB (416)</a></html>) work properly as shown by the expression of eGFP (green, second panel). The red color (third panel) is due to autoflourescence of the plastides and indicates their localisation within P. tricornutum. The very left and the very right panel represent DIC and an overlay of green and red, respectively. |
<html><img src="https://static.igem.org/mediawiki/2013/1/12/416_496.jpg" alt="eGFP in P. tricornutum" /></html> | <html><img src="https://static.igem.org/mediawiki/2013/1/12/416_496.jpg" alt="eGFP in P. tricornutum" /></html> | ||
Revision as of 17:57, 12 October 2013
eGFP
the fluorescent protein eGFP, codon usage of E. coli, works also in Phaeodactylum tricornutum. The excitation peak is at 488nm and the emission at 507nm.
Usage and Biology
In the following picture we demostrate that both promotors (i.e. PNR (496) and PfcpB (416)) work properly as shown by the expression of eGFP (green, second panel). The red color (third panel) is due to autoflourescence of the plastides and indicates their localisation within P. tricornutum. The very left and the very right panel represent DIC and an overlay of green and red, respectively.
eGFP localisation in P. tricornutum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]